Mouse oocyte meiotic resumption and polar body extrusion in vitro are differentially influenced by FSH, epidermal growth factor and meiosis-activating sterol

Meiotic spindle configuration is differentially influenced by FSH and epidermal growth factor during in vitro maturation of mouse oocytes.

BACKGROUND To ascertain whether different hormonal treatment protocols could affect metaphase II (MII) spindle morphology, meiotic spindle organization was detected in prepubertal mouse oocytes matured under conditions allowing spontaneous, FSH- or epidermal growth factor (EGF)-dependent meiotic maturation. METHODS Oocyte-cumulus complexes (OCCs) were matured either spontaneously (control; n=...

NPR2 is involved in FSH-mediated mouse oocyte meiotic resumption

BACKGROUND Previous studies have reported that follicle-stimulating hormone (FSH) is often added to culture media to induce oocyte meiotic resumption and maturation and to improve subsequent embryonic development during in vitro maturation (IVM). However, the underlying mechanisms remain unclear. METHODS Cumulus-oocyte complexes (COCs) were collected from ovaries 46-48 h after the female mice...

Effect of Fibroblastic Growth Factor on Resumption of Meiosis, In Vitro Maturation And Embryo Development of Immature Mouse Oocytes

Purpose: The purpose of this study was to evaluate the effect of fibroblastic growth factor on resumption of meiosis, in vitro maturation of immature mouse oocytes and resulting embryo development with and without basic fibroblastic growth factor-4 (bFGF-4). Materials and Methods: cumulus – oocyte complex (COCs) and germinal vesicle (GV) were obtained from female NMRI mice 46-48 hours after adm...

Reducing CYP51 inhibits FSH-induced resumption of mouse oocyte meiosis in vitro

Gonadotropin-controlled mammal oocyte meiotic resumption.

Fully grown mammalian oocytes resume meiosis as a consequence of rises in gonadotropin levels at the mid-cycle. The increase of cyclic adenosine 3',5'-monophosphate (cAMP) and the activation of protein kinase A (PKA), protein kinase C (PKC) and mitogen-activated protein kinase (MAPK) in cumulus cells are required for gonadotropins-induced meiotic resumption of oocytes. The various actions of cA...